ABSTRACT
Salvianolic acid is the water soluble extract of Salvia miltiorrhiza,and it can improve the blood circulation of the brain and improve the cognitive disorder of depression,anti-inflammation,anti-tumor and so on.At present,the research on its technology is relatively less.This particle will mainly review the research progress of analytical methods,extraction and purification technology of salvianolic acids.The main analysis methods used for salvianolic acids include UV spectrophotometry,near-infrared spectroscopy,quantitative analysis of multi-components by single marker (QAMS),colorimetry and so on.The extraction process is mainly heating reflux extraction and warm soaking method,percolation method,enzymatic extraction,etc.The purification process is macroporous resin purification,ZnCl2 precipitation method,ultrafiltration and so on.
ABSTRACT
Salvianolic acid is the water soluble extract of Salvia miltiorrhiza,and it can improve the blood circulation of the brain and improve the cognitive disorder of depression,anti-inflammation,anti-tumor and so on.At present,the research on its technology is relatively less.This particle will mainly review the research progress of analytical methods,extraction and purification technology of salvianolic acids.The main analysis methods used for salvianolic acids include UV spectrophotometry,near-infrared spectroscopy,quantitative analysis of multi-components by single marker (QAMS),colorimetry and so on.The extraction process is mainly heating reflux extraction and warm soaking method,percolation method,enzymatic extraction,etc.The purification process is macroporous resin purification,ZnCl2 precipitation method,ultrafiltration and so on.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of selenium and zinc alone or in combination on the growth and proliferation of human esophageal cancer Eca109 cell line in vitro.</p><p><b>METHODS</b>Different doses of sodium selenite and zinc sulfate were added into the culture medium of Eca109 cells and normal liver epithelial HL7702 cells (control), and the changes in the cell growth were assessed by means of cell growth curve, (3)H-thymidine incorporation assay and flow cytometry.</p><p><b>RESULTS</b>High-concentration selenium (0.3 micro g/ml) and zinc (3.5 microg/ml) alone both obviously inhibited Eca109 cell proliferation, and the inhibitory effect was enhanced by a combined treatment. At high concentrations, both selenium and zinc promoted HL7702 cell proliferation, but when combined, they produced inhibitory effect on the cell growth. Selenium and zinc at the physiological concentrations ( 0.1 microg/ml and 1.0 microg/ml, respectively) produced similar effects on Eca109 cells and the control cells. Selenium at 0.3 microg/ml caused Eca109 cell growth arrest in S phase, but this effect was not statistically significant; 3.5 microg/ml zinc significantly increased the number of Eca109 cells in G(1) phase. When combined, 0.3 microg/ml selenium and 3.5 microg/ml zinc caused significant G(1) arrest and promoted apoptosis of the cancer cells, an effect stronger than that of either of the agents used alone.</p><p><b>CONCLUSION</b>High-concentration selenium and zinc show a synergetic effect in inducing growth inhibition of human esophageal cancer Eca109 cell line possibly by causing cell cycle arrest and promoting cell apoptosis, and their combined use can be toxic to normal human liver epithelial cells.</p>
Subject(s)
Humans , Apoptosis , Carcinoma, Squamous Cell , Pathology , Cell Line, Tumor , Cell Proliferation , Dose-Response Relationship, Drug , Drug Synergism , Esophageal Neoplasms , Pathology , Selenium , Pharmacology , Zinc , PharmacologyABSTRACT
Objective To investigate the clinical characteristics,diagnosis,treatment and prognosis of human swine streptococcosis occurred in some areas of Jiangsu Province from late summer to autumn since 1998.Methods The epidemiologic and clinical features of 42 cases were collected and analyzed.The bio- chemical features of strains isolated from patient's blood or cerebrospinal fluid(CSF) were tested,and the homogeneity were compared among 15 Streptococcus suisⅡ.Results All patients had acute infection toxe- mic symptoms such as chill,fever,headache and malaise etc.Toxic shock syndrome or meningitis syndrome were the major clinical manifestations.Forty two cases of human swine streptococosis were classified into 3 types:the rates of general,shock and meningitis type were 7.1% (3/42),38.1% (16/42) and 54.7%(23/42),respectively.Ten patients were died of shock type,32 were cured.Strain isolated from patients was identified as Streptococcus suisⅡby API-Strep,the biochemical reactional code was 0641473,and appraised result was 99.9%.There was highly homogeneity in the strains of Streptococcus suisⅡisolated from patients and sick pigs identified by genomic fingerprinting.Com- bined therapy of large doses of penicillin G and ceftriaxone was effective in these patients.Conclusions Human swine streptococosis is zoonosis caused by Streptococcus suisⅡand the clinical manifesta- tions are variable.In the cases of shock type,the onset of disease is stormy and the fatality rate is very high.While the prognosis of general and meningitis type is good and the majority of the cases are cured by effective antibiotic therapy.